FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134426204
Sequences flagged as poor quality0
Sequence length89
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1681090.12505671885222616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1666510.12397210889031725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT9129500.072.605231
AGCAGTG9959000.066.540432
GCAGTGG12345550.053.729813
AGTGGTA12418750.053.2561455
CAGTGGT12500100.052.988824
GTGGTAT12974000.050.9366386
TGGTATC13448700.049.162797
GTATCAA13566300.048.776859
GGTATCA13646650.048.4806448
CAACGCA13186950.028.15278812-13
ATCAACG13213750.028.083610-11
ACGCAGA13559550.027.38224414-15
TACATGG13334350.027.33133122-23
GCAGAGT13626950.027.11798516-17
AGTACAT13567150.026.96588520-21
AGAGTAC13733900.026.8198618-19
CATGGGG7548950.025.6904724-25
CATGGGA4364400.025.51575524-25
TCAACGC13185650.025.14456612-13
AACGCAG13463350.024.63578814-15
Produced by FastQC (version 0.11.5)