FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74369047
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAAT696350.06.6617371
CAAAAAT765400.06.58884331
CTAAAAC323900.06.15251641
AAAAATC684400.05.91277032
CGAAAAT87450.05.911951
AATCTAG190100.05.88427165
AATTAGC284350.05.8016545
CTTAAAC248650.05.78403431
CTAAAAT462850.05.77789831
AATCCAG565200.05.7627395
AAAAATT1124050.05.74511862
CTGAGAT694950.05.74185231
AAATCCA656200.05.73712354
AAATCTA363150.05.73344374
CTTAAAT353350.05.7195421
AAAATCC530750.05.67630773
CTGAAAC590600.05.64222861
CAAAAAC612400.05.6178971
TGAAATC505700.05.5950172
GAAAATC516650.05.5946972
Produced by FastQC (version 0.11.5)