FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46271610
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG246850.071.7042894
TAAGTCG126600.063.44628594
GAAGTCG260950.055.20406394
AAAGTCG125600.051.30334594
GTCGGTT62600.023.8753991
CGGGTTC270700.021.841893
CCGGGTT286500.021.3099482
CCCGGGT306450.020.9502351
ACAAGTC319750.018.77060394
CGAAGTC132650.017.04259394
GCAAGTC306800.015.70241294
ATAAGTC166250.015.60541594
TCGCTAT81550.015.272842
GGTTCAA608550.014.7823515
CTCGCTA88650.014.7388611
GGGTTCA578000.014.6204154
CGCTATG80750.014.6092883
CAAGCGA311250.013.5752619
TCAAGCG282200.013.4404688
CTCGGGT98200.013.2097771
Produced by FastQC (version 0.11.5)