FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46271610
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG307000.066.611494
GAAGTCG270350.058.01331794
AAAGTCG144300.052.50450594
TAAGTCG69100.041.62662594
ACAAGTC316100.019.92407494
CGAAGTC106150.018.8177194
GCAAGTC346500.017.36219494
CCAAGTC462500.016.96064894
AGAAGTC484850.015.96555694
GGAAGTC385500.013.837872594
CGCAAGT91350.011.679255592-93
GAGATAC216400.011.1853053
GGGAGAT364350.010.7583361
CAAAGTC399900.010.69517494
CCGAAGT138500.09.9429694
TCACGAA147450.09.86537814-15
ATACCAT237700.09.807326
CACAAGT307600.09.79421394
GATACCA248000.09.5516135
AGCGCGT113950.09.4041249
Produced by FastQC (version 0.11.5)