FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46202240
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG487480.10551003587704838No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG478270.10351662603371613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG108900.063.9182794
CAAGTCG98700.032.09523894
TAAGTCG67000.030.7955294
CGTACGG14400.026.111113
GTCCGTT37150.025.682371
GTCCGAT25450.024.9312381
GCGTACG15350.024.4951152
GTCGGTT33800.021.4142021
GAAGTCG99150.021.14170594
CGTATAC148600.020.87483294
GTCGAGT178400.020.0619422-23
GTCCGCT62200.019.4196151
CAGTCGA185450.019.41331920-21
CCGATCG185850.018.42319174-75
ATCGCAG196950.018.31556346-47
AAATCGC199450.018.27450644-45
CCCCACT475250.018.1275121
TAGCGCA194200.017.94567584-85
AGTCGAG187550.017.72993922-23
GTCCGTA29850.017.6348421
Produced by FastQC (version 0.11.5)