FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46202240
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG123000.055.40650694
GGGCGAT72400.036.8080141
GGCGATC76050.035.1650242
CAAGTCG118450.033.37019794
GGTCGAC49600.031.838711
CGATCTG100550.026.783694
GTCGACC60650.025.572962
GAAGTCG105750.024.094
GGCGCGT75900.023.716734
CGACCCG65850.023.4821574
CCGTGCG71500.021.9552448
CGTGCGG72500.021.457939
TCGACCC75650.021.0614663
CGCGTGC89450.020.2291776
GCGATCT139800.019.6673833
TGCGACA84250.019.19050412-13
TAAGTCG39950.018.23529494
TGGCGCG100000.018.0953
GCGCGTG103150.017.7246745
ACCCGTG94150.017.0228376
Produced by FastQC (version 0.11.5)