FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44240974
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG307900.071.99007494
TAAGTCG109750.038.26990594
CAAGTCG205500.035.8246294
GAAGTCG298400.032.68533394
AAAGTCG126850.021.59247894
GTTTTTT804750.019.1418151
CGAAAAT120800.017.989521
CACTCGT93500.016.63185794
GTAAAAC209900.016.5919321
CGCAAAT53050.015.8370081
CGTATCG67600.015.78109650-51
TTTTTTC1074350.015.3911772
ACTCGAA182950.015.35653394
TTTTTTG1136050.015.2906742
ATTAGCG35350.015.2757476
TTTTTTA1219800.014.7179682
TTAGACG68950.014.506878
CGTAGCG82600.014.48661130-31
GTTTAAT328800.013.9608741
GGTCGTA91150.013.78192126-27
Produced by FastQC (version 0.11.5)