FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44240974
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG318350.067.7083494
CAAGTCG244500.035.49445794
TAAGTCG101950.034.99953594
GAAGTCG277250.031.30169394
AAAGTCG113500.020.79292594
CTCGGTT150850.017.6291281
GTTTTTT910800.017.3176141
CGAAAAT103850.016.2875481
GTAAAAC206800.016.0630721
TCGGTTT175150.015.5046572
CGTAGCG70050.014.2575330-31
CGATAAC40400.013.9559561
GTTTAAT360650.013.9007491
CGTATCG70950.013.67368750-51
TAGCGAC80450.013.67158780-81
CTCGTTT70800.013.6707921
CGGTAAG73400.013.2543149
GTTTTCT621850.013.1544551
GTACGAT40750.012.9173489
CGTGATA65150.012.6964199
Produced by FastQC (version 0.11.5)