FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70659755
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG133400.059.8245994
CAAGTCG298850.048.58055594
AAAGTCG194400.040.85905594
GAAGTCG399450.038.41657694
ATAAGTC202500.012.32444494
ACAAGTC374750.010.64789994
CTAAGTC214050.010.45176394
GCAAGTC418300.010.14606794
TTAAGTC223750.09.15843694
CGAAGTC225400.08.882874594
GGAAGTC648250.08.64234494
AGAAGTC685500.08.20014694
CCAAGTC623300.07.849671494
GTAAGTC287450.07.68481594
TCAAGTC424400.07.508482594
TGAAGTC675250.06.403554494
GAAAGTC427400.05.872250694
AAAAGTC441650.05.35288194
CAAAGTC601900.05.31766194
GCGAAGT130950.05.18633192-93
Produced by FastQC (version 0.11.5)