FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39168484
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86500.049.0647494
CGACGGT46300.046.1879041
GTATCGA56800.038.8908466
CGGTATC56300.038.6518634
GGTATCG57100.038.1926465
GACGGTA58850.036.2582862
TATCGAT61650.035.9075437
ATCGATA61950.035.7336588
ACGGTAT62950.034.3447153
TCGATAA66800.033.491029
TAAGTCG59750.027.29539994
GATATCG43350.025.3702435
CGCTCTA68700.025.03930352-53
TTAACCC175300.024.3177412
TATCGGA48750.023.52410360-61
CTATCGG48700.022.72792660-61
CGATTGA55450.022.631290-91
ATATCGA48450.022.6026826
TCTATCG51950.022.30125258-59
TATCGAA49700.022.1287737
Produced by FastQC (version 0.11.5)