FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46114401
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG139700.062.8797494
CAAGTCG115250.033.0733294
TAAGTCG45450.029.67876894
CGTCGGA103450.024.94248494
GAAGTCG171250.023.82248394
GTTCGAT94800.022.8554861
TTCGATT113300.019.4969122
GGTTCGA93300.019.1929264
CGGTTGT182950.019.139113
TCGATTC110850.018.4862423
AATAGCG85250.018.27624790-91
GTCCGAT23600.017.127121
CGAATGT300200.016.110268
CGCTGTT211700.015.6962681
GTTCCGT121950.015.47396524-25
TAGCGGC98650.015.15053292-93
TCCGTTG126400.014.96637626-27
TCGAATG327300.014.8768717
ATCGGTA34000.014.6529416
GGGTTCG136600.014.6229873
Produced by FastQC (version 0.11.5)