FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46114401
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG150800.058.99933694
CAAGTCG121700.032.59490694
TAAGTCG46000.030.65217494
GTTCGAT88900.022.9448811
CGGTTGT174800.022.2631573
GAAGTCG178000.020.99157394
AATAGCG83900.020.22288390-91
CGTCGGA119100.020.16540794
TTCGATT109000.018.9724792
TCGATTC109950.018.0818563
GGTTCGA92400.017.9556274
CGCTGTT198450.017.691611
GTCGGTT214500.017.3976691
CGAATGT281300.016.8585158
TAGCGGC94950.016.8299192-93
GTTCCGT115350.015.9111424-25
TCCGTTG118100.015.64013626-27
TCGAATG310700.015.3540397
CTTATCG33750.015.3185193
AAAGTCG67300.014.87518694
Produced by FastQC (version 0.11.5)