FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46260596
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA877810.1897532837665991No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT643800.1391681162084466No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG615540.1330592454969668No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG530990.11478235170165124No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG507640.10973485944711997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTCG222750.042.4740794
CAAGTCG297450.041.06673494
AAAGTCG168350.022.97653894
GAGTACC187250.022.9164246
CGGAGTA148200.022.3583014
CGAGTGT313350.021.8388393
TAAGTCG292400.021.5068494
GTGTCCG338400.021.1666666
TGTCCGG357650.020.1588157
CCGAGTG335250.020.0337072
CGGACTA251200.019.645782-83
GTACCTT228000.019.3771938
AGTACCT226850.019.0403357
GTCGGTT196600.018.909971
CTACGAC158900.018.26463386-87
TCGCTAT111150.018.140352
CCCGTTA135700.018.0103171
AACGTAT425750.017.7623026
GAGTGTC417800.017.2228324
GAACGTA453250.017.1512415
Produced by FastQC (version 0.11.5)