FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46260596
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG891070.19261965410043572No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA541460.11704561696524618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG383850.034.0515894
AAAGTCG137700.032.01597694
GCGCACG422950.024.1250726
GAGATAC276500.024.035443
TGGCGCA448400.022.5147194
GCACGCC451100.022.5049888
CGGTGGC494750.021.7259221
GGCGCAC503050.020.8349085
CGCACGC498350.020.673027
GGGAGAT367600.020.6360171
GAAGTCG524650.020.1294294
CACGCCT537750.019.3069279
ATACCAT351050.018.489396
GATACCA356700.018.4468735
TAAGTCG135700.018.01031794
CGAAGTC118750.016.54399994
TACCATG415250.015.7553277
GGTGGCG691050.015.3844152
ACCATGA428400.014.8877228
GTGGCGC743900.014.4683433
Produced by FastQC (version 0.11.5)