FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49987166
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA647620.12955725475615082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG201150.068.1575994
TAAGTCG93400.067.43040594
GAAGTCG192750.053.25447594
AAAGTCG119050.050.17807494
CTATCGG84650.023.4305956
CCCGGGT208150.022.8734071
CGGGTTC161550.022.6926653
CCGGGTT175850.021.8896792
TCTATCG90850.021.7281235
TATCGGG96700.020.2192347
GTCCCCC442400.017.7418611
GTCCGGT41700.016.6810551
GTCGGAT28250.015.9716821
CTCGGGT101950.015.720451
TATACCC222150.015.65608794
GTCGGGT49200.015.475611
TCGTATA124650.015.13878890-91
TCGAGTT381100.015.03975326-27
ATCCGGA366450.014.81375366-67
GTCGGGA118250.014.7458771
Produced by FastQC (version 0.11.5)