FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66959966
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG207800.072.9653594
CAAGTCG448050.060.84142394
AAAGTCG276900.055.113494
GAAGTCG532150.047.54317594
CTAAGTC225450.020.01330694
ACAAGTC406600.019.23462794
ATAAGTC203400.019.04031494
GCAAGTC455850.015.79554794
TTAAGTC208900.015.77166294
GTAAGTC273500.013.71334694
CCAAGTC685150.013.32861494
GGAAGTC683700.013.02691294
TCAAGTC441700.012.98166294
AGAAGTC691700.012.74714594
CGAAGTC269500.012.71354394
GAAAGTC389200.011.03751294
CAAAGTC600350.010.31831494
AAAAGTC397200.010.17623494
GATCGTA59750.09.91129794
TGAAGTC660100.09.88274594
Produced by FastQC (version 0.11.5)