FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50678157
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA790800.15604355935832473No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT532210.10501763116602682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG166900.060.91132494
TAAGTCG74900.060.67957394
GAAGTCG173950.047.22966894
AAAGTCG102900.038.5500594
CTATCGG141300.027.8740276
TCTATCG144650.027.4234355
TATCGGG157350.025.0009547
TATACCC245600.024.7630394
CGGGTTC228550.023.3817563
CCGGGTT247100.022.8437882
CCCGGGT245450.021.1973931
TCGCGCA48500.020.93195794
GTCGGGT55150.019.8567521
CACTACC456450.018.637317
ATCGGGG210950.018.3811328
GTCGATT40550.018.081381
CGTATAC189850.017.24282592-93
ATCCGGA330700.017.16132566-67
TCGAGTT341300.017.06211526-27
TCCCCCA538800.016.8530062
Produced by FastQC (version 0.11.5)