FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50678157
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA621600.12265639415419151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG190200.066.1014794
GAAGTCG175650.046.66552794
AAAGTCG100250.041.72568594
TAAGTCG42250.038.8236794
GGGCGAT98650.025.0126721
GGCGATC109800.022.6010932
GAGATAC325350.021.2355923
AGATACC341100.020.1172684
GGGAGAT423000.019.2666661
GATACCA356600.019.190135
GGAGATA369800.019.1024882
ATACCAT384050.017.7450856
TGCGACA125850.015.31187912-13
ACAAGTC300900.015.10435394
TCAATCG44950.014.8476093
TCACGAA320050.014.5530414-15
TACCATG470050.014.4984577
CGAAGTC86700.014.41983994
ACCATGA488350.013.6760518
ACGAAGG347950.013.52794916-17
Produced by FastQC (version 0.11.5)