FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49009016
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA608140.12408737200518369No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG548510.1119202230054976No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC492000.1003896915620587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG215850.070.39657694
TAAGTCG93850.064.4027694
GAAGTCG229800.051.54046694
AAAGTCG113750.050.8219894
CGGGTTC266050.026.4457823
CCCGGGT302550.026.2069051
CCGGGTT284900.025.7848382
TCGCTAT97950.020.680962
TATACCC231400.019.86430594
GTCGGTT51350.019.5871491
CTATCGG102050.019.0671236
CTCGCTA108450.018.938681
TCTATCG101550.018.8833085
CGCTATG104050.018.7909663
TATCGGG103700.018.0838977
ACAAGTC316850.017.63705394
CGAAGTC110800.017.22202194
GGGTTCA602250.016.9738464
TCGTATA148900.016.49261390-91
GGTTCAA629700.016.2787065
Produced by FastQC (version 0.11.5)