FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49009016
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA561930.11465849467371472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG249150.068.92956594
GAAGTCG222200.057.3010894
AAAGTCG112950.051.34838594
TAAGTCG57950.047.44607594
GAGATAC301400.021.098543
ACAAGTC293950.020.11430794
GATACCA322700.019.516585
AGATACC330700.019.1865734
GGGAGAT438300.018.5404971
ATACCAT332100.018.4122246
GGAGATA374350.017.5896872
GCAAGTC339550.015.98733694
CGAAGTC101300.015.82132294
TACCATG425450.014.5159247
GGCGATC89800.014.3407571
CCAAGTC462350.014.323132594
AGAAGTC463950.014.01034594
GGCGCGT218350.013.7975731
ACCATGA438050.013.7443228
TCACGAA319850.013.62169714-15
Produced by FastQC (version 0.11.5)