FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49728645
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA525880.1057499153656811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG199350.068.5372594
TAAGTCG85250.065.1108594
GAAGTCG207750.051.26450394
AAAGTCG118850.048.24568694
CGGGTTC197200.023.762173
CCCGGGT242700.022.8706231
CCGGGTT224850.021.6553272
CTATCGG90150.020.437056
GTCGGTT38750.019.5277421
TATCGGG98000.019.1357157
TCTATCG97000.018.945365
TATACCC206600.017.74443494
TCGCTAT80250.016.8672892
GCCGGTA58750.016.481
CTCGGGT98600.016.3975661
CTCGCTA81000.016.0148161
ACAAGTC318950.015.6642194
CGCTATG84600.015.4444453
CTCCGGT67400.015.4109781
TCGTATA129850.015.38313590-91
Produced by FastQC (version 0.11.5)