FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45418247
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG152950.045.72474794
CAAGTCG141250.024.72283294
TAAGTCG42600.019.85915694
GTCGGTT87450.017.6283591
GTTCGAT45800.013.5458521
GTCGGAT50800.012.4901571
GAAGTCG211900.012.08824994
GGCGATT88700.011.4983081
GTCGGAA76550.010.6832131
AAAGTCG84600.010.66666794
GTCCGAT48050.010.6618111
TCGGTTG104650.010.2847592
CGGTTGT129100.010.2300553
GGCGGTT101500.09.9093591
GTCGGGT70950.09.8703311
GCCGGTT99750.09.8476181
GGGGAAT255500.09.8414871
CTCGTAT39400.09.7817261
CGCGAAT16050.09.6635511
GGGGGAT266300.09.5835521
Produced by FastQC (version 0.11.5)