FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45418247
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG176100.038.99318394
CAAGTCG152550.023.78498894
TAAGTCG46300.017.7645894
GTCGGTT84850.015.454331
GGCGATT85350.011.8394841
GTCCGAT50100.011.8203591
GTTCGAT46800.011.5491451
GAAGTCG219900.011.2851394
GGCGGTT101350.011.2224961
GGCGGAT187050.010.6789641
GGCGAAT65400.010.63608551
GCCGGTT91800.010.5468421
GTCGGAT52300.010.514341
GCGGGTT99900.010.2562561
AAAGTCG82550.010.02059494
GCGGGAT115400.09.9783361
CGGTTGT117500.09.843
CGCGGAT57300.09.7609071
GGCGTTT101650.09.6635511
CGGGTAT47350.09.5290391
Produced by FastQC (version 0.11.5)