FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46204167
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG139350.063.94833494
CAAGTCG125800.031.83147694
TAAGTCG44700.027.44295394
CGTCGGA155600.022.26156894
GAAGTCG175850.020.74040494
CGACCGT23000.020.0260871
GTCCGAT35900.019.3760451
GGTTCGA104200.018.5383874
AATAGCG88900.018.37176590-91
GGCGGAT260250.016.8134481
GTTCGAT108750.016.8119545
GGGTTCG132700.015.7611153
TAGCGGC102800.015.56760892-93
CGGATGA227750.015.2092213
TTCGATT124450.014.9554026
CGTATCG79150.014.904613
GTCGGAA146700.014.7695971
AAAGTCG61750.014.76599294
CGAATGT250800.014.7109248
TCGATTC130450.014.2675347
Produced by FastQC (version 0.11.5)