FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50178215
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG254000.074.4783594
GAAGTCG246750.059.4285794
TAAGTCG141300.053.95187894
AAAGTCG139550.052.87710694
GTTCGGG90650.028.360731
CCCGGGT254150.026.7224081
GAACGTA217400.023.4999984
CGTAAGG132250.023.2068067
CGGGCAT227700.021.9003091
ACGTAAG142900.021.5759286
TCGGGTC115400.020.771233
AACGTAA156550.020.4152035
CCCCGTT146300.019.5967181
GTCGGTT72100.019.5561711
CGGGTTC291100.019.2940563
CCGGGTT308700.018.8487222
AGAACGT165050.018.822783
ACAAGTC373400.018.4400194
TTCGGGT135900.018.0183962
GGCGTTT144700.017.7671051
Produced by FastQC (version 0.11.5)