FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50178215
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG306900.068.9762194
GAAGTCG282200.056.7430994
TAAGTCG76850.051.18932794
AAAGTCG148150.047.6186394
ACAAGTC337200.020.21055894
CGAAGTC105150.018.23680394
CGTTTTA149950.017.8032689
GCTCCGT156500.017.4785945
TAACGCA141100.017.32104994
AGAAGTC522800.017.19797194
CTCCGTT158650.017.1232286
GCAAGTC357750.016.05422694
TCCGTTT174200.016.0533877
CCGTTTT178500.015.4823528
CCAAGTC506900.015.447227594
AGCTCCG197800.014.0429734
GGGAGAT416700.013.4221261
GAGATAC300350.012.4717833
CCGGTTA132650.011.9581654-55
GATACCA310000.011.8106465
Produced by FastQC (version 0.11.5)