FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50120175
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA1077300.2149433835775713No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG869690.1735209424149058No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT533340.1064122381855211No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG517370.10322589655762375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG179250.064.3185494
TAAGTCG79200.058.2752594
GAAGTCG195650.046.0751394
AAAGTCG115800.041.76424894
GTCGGCT85750.023.130031
CGGGTTC193350.021.2940253
CCCGGGT235150.021.0465641
CCGGGTT209700.020.6199342
GTCGGTT50050.019.9080921
TCGCTAT79300.018.4325352
CTCGCTA80800.017.6831681
CGAGTGT185650.017.4683553
CTAGGCG88650.016.7535259
GTGTCCG200300.016.4488286
CGCTATG85700.016.3430563
TCGGCTG122000.015.9106562
CTCGGGT106700.015.7253981
TGTCCGG210750.015.2763937
GTTCGGA78600.015.1882951
TCGTATA97850.015.08226990-91
Produced by FastQC (version 0.11.5)