FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50120175
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG883000.17617655963890788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG220650.059.64196894
GAAGTCG181850.051.09650894
AAAGTCG110350.044.2102494
TAAGTCG51300.042.51072394
TGGCGCA327700.029.2441254
GCGCACG330150.029.0982886
CGCACGC371050.026.0175197
CGGTGGC418800.025.7893981
GGCGCAC426000.024.0626755
GCACGCC456950.021.239748
GGTGGCG528450.020.1536562
GTGGCGC541500.019.7113573
CACGCCT685500.014.8370529
ACAAGTC298150.014.69193494
GGCGATC72900.014.4417011
GCAAGTC296900.013.45570994
AGCGCGT75300.012.8579029
GAGATAC223250.012.4421053
CGAAGTC88350.012.34182394
GGGAGAT352550.011.4783711
Produced by FastQC (version 0.11.5)