FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50322964
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG271850.071.62810594
GAAGTCG241550.061.77809594
AAAGTCG123950.051.18999594
TAAGTCG68000.045.13382394
ACAAGTC346600.019.62175494
CGAAGTC98900.018.01112294
GCAAGTC347200.015.7704594
CCAAGTC497600.015.28255794
AGAAGTC548950.014.38382394
GAGATAC262800.012.3044143
GGGAGAT408050.011.9443691
GGAAGTC414100.011.78121294
TCACGAA188350.011.40376914-15
AGATACC303250.011.2211054
GATACCA291700.011.1498125
CGCAAGT73300.010.6118792-93
ACGAAGG203250.010.49840116-17
ATACCAT300750.010.4392366
TCCGGAT200950.010.26772856-57
TGCGATT210950.09.92581274-75
Produced by FastQC (version 0.11.5)