FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36228588
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2005640.553607002293327TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG900970.2486903436589911TruSeq Adapter, Index 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT407750.037.2005842-43
TATGCCG435450.037.02639846-47
CGTATGC436250.036.92183344-45
ATCTCGT452450.033.45692440-41
CACGATC448650.033.20915236-37
ATCACGA504700.031.69840834-35
CCGTCTT542500.029.83265350-51
TGCCGTC554000.029.38338948-49
CGATCTC544750.027.67224338-39
TCGTATG416450.027.59419842-43
CGTCTGA624850.026.08729616-17
TCACATC628550.025.3270830-31
CTTGAAA642550.024.96948260-61
CACGTCT649900.024.96091814-15
TGCTTGA658800.024.63972558-59
TCTCGTA437400.024.54552740-41
ACATCAC660100.024.1570132-33
ACGATCT440100.023.96350736-37
TCACGAT471700.023.73164734-35
TCTGAAC690250.023.72234318-19
Produced by FastQC (version 0.11.5)