FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42729378
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124950.054.39135494
TAAGTCG81400.032.33415294
CGACGGT47600.032.1890751
GTATCGA49900.031.0821636
CGGTATC53450.029.3695054
GGTATCG55850.028.0232755
TATCGAT57050.027.2690627
GATATCG50600.027.2154145
ACGGTAT57600.027.1718733
GACGGTA57750.026.9385282
ATCGATA58550.026.8112728
TCGATAA59900.026.4424049
TATCGAA54250.025.5576047
ATATCGA54200.025.3210326
TTAACCC185250.021.8699052
CGCTCTA73450.020.9564352-53
CGATTGA51750.020.84347790-91
ATTAACC207400.019.9421391
TAACCCT207650.019.6012523
TCTATCG45400.018.63436158-59
Produced by FastQC (version 0.11.5)