FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42729378
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG149750.045.10116694
CGACGGT51550.035.9224051
GTATCGA53250.034.8638466
CGGTATC58600.031.6808874
GGTATCG60250.031.125315
ATCGATA60800.030.6891448
TATCGAT60550.030.660617
GACGGTA63250.029.5747012
TCGATAA64100.029.255859
ACGGTAT64050.028.8384063
TAAGTCG85950.028.3257794
GATATCG49750.024.9407025
TATCGAA53350.023.9625117
ATATCGA55300.022.6925856
TTAACCC186650.021.6555062
ATTAACC203250.020.5343171
CGCTCTA71750.020.24111452-53
TATCGGA49750.019.69748760-61
CGATTGA48550.019.65190390-91
CTATCGG48450.019.54695560-61
Produced by FastQC (version 0.11.5)