FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48328388
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG183000.066.8016494
CAAGTCG152950.034.6930494
TAAGTCG71700.028.973594
GAAGTCG212500.024.39576394
GTAAAAC241150.021.1660791
AAAGTCG78900.020.7300494
TAGCGAG95250.020.5763788
TTAGCGA109250.018.240737
ACTCGAA240650.017.92894294
CACTCGT98400.017.38617994
TCTCGTG234500.017.0963765
CGCTAAT38500.015.99220851
GCGAGGT149950.015.922641
CGCAAAT61450.015.908871
CGTAGCG114450.015.4818730-31
GTTTTTT1303100.015.4550681
TACGGAC99800.015.44689494
CGAAAAT212500.015.0178811
CGAGGTT144900.014.9855072
GTATCGA86650.014.83496852-53
Produced by FastQC (version 0.11.5)