FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48328388
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG210350.057.42334494
CAAGTCG168150.031.9203194
TAAGTCG75300.029.08631994
GAAGTCG213200.022.37570494
AAAGTCG87200.021.61353194
CACTCGT92800.019.14439894
GTAAAAC249800.019.0408331
TAGCGAG87100.018.562578
CGCAAAT59300.017.516021
TCTCGTG230500.016.9648595
CGAATGT131050.016.8920271
TATCGGG109650.016.5882345
ACTCGAA245800.016.50162794
CATATCG112100.016.351473
TTAGCGA103100.016.137737
ATTAGCG36950.015.3910696
GCGCTTC230600.015.3881182
ATATCGG132200.015.1096824
TACGGAC114200.014.98073694
CGTAGCG108000.014.70925930-31
Produced by FastQC (version 0.11.5)