FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61489520
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG186800.071.53158694
CAAGTCG296750.060.29620494
GAAGTCG325000.045.8575494
AAAGTCG186450.044.5674494
ATAAGTC282300.020.8278494
ACAAGTC420800.016.44106594
GTAAGTC296100.011.61904794
AGAAGTC658200.011.46080294
CTAAGTC225250.011.18401794
GCAAGTC360400.010.94145394
TTAAGTC255850.010.06683594
TCAAGTC461800.09.05803394
GGAAGTC542850.09.01298794
CCAAGTC528200.08.4888394
GATAAGT213800.08.28765194
CGAAGTC144600.07.345781394
CATAAGT224350.06.787608694
AAAAGTC498300.06.46096794
GAAAGTC405800.06.439625794
AATAAGT371350.06.34091894
Produced by FastQC (version 0.11.5)