FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49458062
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG170150.037.56685694
TAAGTCG50850.021.81317594
CAAGTCG142750.018.931794
CGCAAAT59250.014.595781
GTTTATT359450.014.1738771
GTTTTTT829900.013.7675621
GTTTTAT470950.013.7023041
GAAGTCG206850.013.33768694
CTCGTTT109400.012.9744051
GTTTTCT657400.012.7044431
GTTTAAT288250.012.63661
CTCAGAT378050.012.220871
CGAAAAT128400.012.0794391
CTTTAAT387700.012.0257941
CTCGATT98300.011.9053931
CTTTATT535850.011.8585421
CTCGGAT149550.011.7225011
CTCGCAT139450.011.6615281
CTCGTAT52950.011.3616631
CGGAAAT118450.011.34824851
Produced by FastQC (version 0.11.5)