FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49130312
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG602770.12268800572648511No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA559180.11381568266857334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG135450.059.3355594
TAAGTCG63650.057.44854494
GAAGTCG170800.039.32259894
AAAGTCG89350.036.6110894
CTATCGG94800.028.2099156
TCTATCG96700.027.4126155
TATCGGG118000.022.743227
TATACCC209450.022.7314494
CGGGTTC178000.021.0443823
CCCGGGT217900.020.3184931
CCGGGTT194250.020.0581722
GTCGGTT41300.018.094431
CGTATAC140650.017.86100292-93
TCGTATA143800.017.81293590-91
TCGCTAT65350.017.6205042
CTCGCTA70150.017.2188171
CTCGTAT152900.016.44538790-91
ACTCGTA160650.016.2371688-89
TACGTTC164350.016.18618826-27
CGCTATG71850.015.961033
Produced by FastQC (version 0.11.5)