FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48845858
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG150050.061.20493794
TAAGTCG72450.055.33609894
GAAGTCG186400.040.49463394
AAAGTCG96000.038.77594
GTCGGTT42850.022.7047861
CTCGCTA73850.021.5748141
TCGCTAT74900.020.3311082
CCCGGGT204300.020.1987291
CGCTATG75950.019.8025043
CGGGTTC155450.019.4712143
CCGGGTT197850.016.1774062
GCGGTAT38400.015.78906251
CTCGGGT93450.015.289461
GTCGGAT29100.014.5360831
GTCGGTC57900.013.880831
TATACCC192100.013.84799794
GTCCGTT33550.013.5886741
GTCGGGT45950.013.5016321
TACGTTC108300.013.43167126-27
CGTATAC105450.013.32669692-93
Produced by FastQC (version 0.11.5)