FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48845858
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG177150.060.80948394
GAAGTCG154800.051.766894
TAAGTCG50150.037.39381894
AAAGTCG92550.037.02107294
ACAAGTC273600.013.81140494
CGAAGTC81150.013.66851594
GGCGCGT123900.012.7836971
GAGATAC243100.012.4121763
GGCGATC72300.012.1562931
TCACGAA165250.012.08774714-15
GGGAGAT378650.011.8911921
AGATACC267400.011.4599864
GCAAGTC283050.011.44073594
GATACCA275000.010.8527275
TCCGGAT180900.010.69126656-57
GCGACAT64750.010.6702717
ACGAAGG188800.010.54263816-17
GGAGATA310300.010.5420562
TGCGATT192950.010.24280974-75
CGCGTGC155050.010.2154153
Produced by FastQC (version 0.11.5)