FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62344009
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT131350.023.6512411
GGCGATC145000.020.6792552
TGGCGCG422650.018.6592253
GGCGCGT498700.016.3132134
GGTGGCG525150.015.5184471
GTGGCGC543250.014.6293842
CGGTGGC427350.014.4618741
CGCGTGC585150.013.9435076
GCGCGTG639650.013.0934615
GCCACGA333250.013.07713418-19
CGATCTG250250.012.0383174
GAGTGCG373200.011.62535128-29
TGCGACA250250.011.57149112-13
ATCGTAG563650.011.3287894
GCGTGCC714250.011.1619787
GCGACAT197650.011.09014312-13
GATCGTA619850.011.07539494
CACGAGC395500.011.04654620-21
TCGTAGC502450.011.01479694
GCGATCT296400.010.9726223
Produced by FastQC (version 0.11.5)