FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49499740
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG272900.081.32429594
CAAGTCG163450.037.611594
GAAGTCG250700.036.89509694
TAAGTCG133300.036.3518494
AAAGTCG109500.027.08401994
CGTCCCG46200.024.7207813
TTCGGCG40800.023.8455899
CCCGTTT87500.019.1761
CCCGTCT120550.018.8701781
CTCGTTT112050.018.623831
CCCGTAT43400.018.5184351
CTCCGTT170300.018.3253081
GTCGAGT77400.017.64018222-23
CTCCGAT56900.017.263621
CTCGGTT77700.016.6344931
CCCGTCC96050.016.3925041
CCCGATT45400.016.0462551
GTCCGTT36100.015.7534641
CTCCTGT541250.015.4741811
CTCCTTT959500.015.4005221
Produced by FastQC (version 0.11.5)