FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49499740
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG299450.073.0936794
GAAGTCG331950.038.31360294
CAAGTCG190650.033.8478994
TAAGTCG79750.025.22382594
CGACCCG45800.025.1419224
GGTCGAC45450.024.4048421
ACGCGCG41850.020.38351432-33
GTCGACC55450.020.3426532
CCGTGCG60050.019.5670288
AAAGTCG101000.019.31188294
TCGACCC61600.019.0746773
CGTGCGG62500.018.49929
GACCCGT71050.017.0668545
GGACGCG53100.016.06497230-31
GCGCGAG57100.015.35113934-35
CGGGACG40800.015.03308990-91
GCGCGGC57050.014.29360376-77
CCGCGCG58250.013.99914274-75
CGCGCGA40650.013.99016134-35
ACCCGTG88150.013.7027816
Produced by FastQC (version 0.11.5)