FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42700007
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG98800.042.4807794
CAAGTCG75200.021.12594
CGTCGGA118450.020.27606694
TAAGTCG34350.018.3347994
GTTGTCG45050.016.5882361
CGCGAGT24950.016.5771541
GTTCGAT93950.015.8584361
GAAGTCG144450.013.66562894
GGGGAAT388750.013.4199351
CGGGTAT30300.013.1848191
AAAGTCG68950.013.01957994
GTCCGAT26100.012.7854411
GTCGGAA113350.012.6881341
GAATGCT269800.012.6645664
GGCGGAT252500.012.6574261
TTCGATT113450.012.3040992
GGGAATG364200.012.1952232
AGCCGTA125400.012.14354182-83
GACATAA173250.012.072155
CGTATCG54500.011.9871563
Produced by FastQC (version 0.11.5)