FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46606388
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG65150.027.485894
TCTCGTG198500.025.358695
CGTGTGA211300.022.5101768
TTCTCGT249500.020.6837654
TCGTGTG237600.020.6712957
CTCGTGT245700.020.5445656
CGCAAAT86800.018.8974671
CGTATCG64550.018.27575550-51
TTTCTCG283600.017.981313
ACTCGAA171850.017.96857694
TCGCGTT28800.017.95138722-23
GTTTTTT1436500.017.4225541
TATATCG56750.016.39823720-21
GTTTTCT815400.016.1739031
GGCGCTT164500.015.6571431
GTCGCGT28450.015.36379522-23
AGTCGCG33750.015.31851820-21
CTCACTT364150.015.1912681
CGCGTTT18400.015.0706521
GTTTAAT406150.015.0437031
Produced by FastQC (version 0.11.5)