FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005788499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46606388
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTG171500.024.9113675
CGTGTGA184150.022.562048
AAGTCGG79050.022.11764794
CTCGTGT213100.020.2247756
TCGTGTG218400.019.4542127
TTCTCGT233450.019.126154
CGCAAAT81650.018.535211
TATATCG51500.017.29417420-21
TTTCTCG268100.016.601643
GTTTTTT1369200.016.5042361
CTCACTT383950.016.0604251
CGTGATA87050.015.981629
ATATCGC58350.015.82776322-23
AACGTAG120100.015.1448796
GTTTTCT801300.015.0449271
GTTTAAT381150.014.2177611
CGTATCG47200.014.18961850-51
CGCAATC149950.014.1673891
GGCGCTT166950.014.1323741
ACTCGAA158050.014.00632894
Produced by FastQC (version 0.11.5)