FastQCFastQC Report
Mon 19 Sep 2022
EGAF00005788622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40148191
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTAT4636151.1547593763315513TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCGCGTAT1671280.41627778447103625TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGTTT939140.2339183850151555TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACAGGAATCTCGGAT813060.2025147284967335TruSeq Adapter, Index 4 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)