FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432854
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG107210.3123057374417904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT7850.092.076129
CGCGATT6850.079.9067469
CCGCATA8850.075.3285759
CGCGATA6700.073.316669
GCGCTAT5000.072.981498
ACCGATA8800.070.1745159
GGCGATA6900.067.123459
ACGCATT7750.066.099869
CGCTATT8250.065.496219
ACGGATA9900.065.212689
GCGGATT7500.062.689239
ACGCGAT3950.062.1799478
CGGTATA8050.061.8930479
ACGGATT10450.061.7804379
TCGCATA7050.061.713769
CGGGATA9900.061.668519
GCGCATA10200.061.23079
GATCGAT3800.060.9410258
GCGCATT9700.060.046239
CGTAATT8200.058.1934979
Produced by FastQC (version 0.11.5)