FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5892606
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA382650.6493731296475618No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG243970.4140273420622387No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG234870.3985842596637209No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT162270.2753790088799421No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC156860.2661980115419222No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT138270.2346500003563788No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG117010.19857088697258904No Hit
TAGATTAAACACTCAACAAAGTAAACATGTTTCTGTCCTCTGTCTCATGT96110.1631027087166527No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC93720.15904677828451452No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT83590.14185574260352718No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA68220.11577220672822855No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC64000.10861068939616869No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG59790.10146614248432698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA13900.0111.166044
GCTAGCC51250.0108.732421
CGACGTA5000.0104.369315
TCGTGTT56550.0102.467384
CGTGTTC57050.0101.688125
CGTGAGT5600.096.79289145
CGATACT6100.096.635867
CGTGTCG3650.096.52771145
GGACGTA5000.093.525745
CGGGTTA4650.093.277975
TAGCGGC19650.092.755278
AAACGCG5050.089.8943259
CGCGAAT1700.087.68297145
AGTCGAA17550.087.659875
CGCACGT7350.087.6228561
TGACCGA26900.087.402959
TAAGCGT5450.087.024796145
TAGCCCT65550.085.607083
TTTCGTG67250.085.36362
TTCGTGT67950.084.877423
Produced by FastQC (version 0.11.5)