FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005790608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2999973
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA167400.5580050220451984No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT122710.4090370146664653No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG96690.32230290072610657No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG74070.24690222211999907No Hit
AGAAAGAATAAGCATGTTACCTTAAGATCAACCACCATGGACTGAACAAG60560.201868483483018No Hit
CAGCACATACACATTTTAGCATACCACAAATTCTTAACCCTTTCATATTC51320.1710682062805232No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAATTGCCT44830.14943467824543755No Hit
CGGCGGCTGGTAAGGGAAGAGGGGCTCCTCACTTCCCAGTAGGGGCGGCC42850.14283461884490295No Hit
CCTGAAGCCACGGGTGTCTTAAGGCTTCTTCTGTCATAAAACGTGCCTTT39860.13286786247742896No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG36660.1222010998098983No Hit
TAGATTAAACACTCAACAAAGTAAACATGTTTCTGTCCTCTGTCTCATGT34820.11606771127606816No Hit
CAGTCAGCCTGCAGATGTGGATCTAATGATTGACTGCCTTGTTTCTTGCC33750.1125010125091126No Hit
CTGGGCTCATGTGACCTACTGCCTTGGCCTCCCAAAGTGCTGGGATTACA33360.11120100080900727No Hit
GGAGAAACAAATGCTGCTCCGATCATCTGCTCTGCTTCTTCTGTTCCTCA31460.10486761047516094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)