FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005790609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005790609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2999973
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGCCAAGACTTCGGGGGGGGGGGGGGGGGGGGGGGGGGG102380.34126973809430955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT5650.085.628199
GACGTAT2700.080.504718
GCGAATT4650.078.40919
GCCGATT6650.071.6973959
CGTTATA5650.070.7363369
CGCGATA6000.068.9472359
GCGCTAT4500.068.5588468
TCGCATT6650.068.534289
ACGCGAT3300.067.992258
ACGTATA5700.067.655639
CCGCATA7800.066.520159
CGGTATA7800.065.621239
GCGCATA8150.065.3840949
CGCGTAT2900.065.281368
AGCGATT10750.065.224049
CGTGATT6100.064.368649
GCGCATT7150.063.7416739
CGATATA6450.063.04999
CGGTAAT6400.062.447968
CCGTATA8300.061.6681449
Produced by FastQC (version 0.11.5)